Q : I would like to change the default settings in the BiNGO Settings Panel. You can use these as custom annotation/ontology files. I would like to use more recent annotations.Ī : Download the most recent annotation and ontology files from the GO website. Q : The default annotations/ontologies in BiNGO are already several months old. However, these genes do have an annotation and they are taken into account. This means that some curator has actually looked at them and had to conclude that there is as yet no clear-cut functional classification. There is however a difference with genes that are annotated to categories such as Biological_Process unknown. The genes for which there is no annotation available are not taken into account in the over-representation analysis, and they're not counted as genes belonging to the test or reference set. Given the current status of GO annotation efforts, a lot of genes haven't actually been looked at by a curator and annotated in the GO hierarchy. Q : The number of genes in my test set according to the BiNGO output window does not match with the number of nodes I pasted into the text field or selected in the graph. Try running Cytoscape from cytoscape.bat (Windows) or cytoscape.sh (Linux, Mac), or see here to increase Cytoscape’s default memory settings. Show Package Contents, which will display the Contents subdirectoryįor example, if you want Cytoscape to initially allocate 2GB of memoryĪnd use up to a maximum of 4GB, edit the Cytoscape.Q : BiNGO aborts without any error message.Ī : Cytoscape’s default memory settings (particularly in version 2.7) are probably not sufficient. To access this inįinder, you will need to right-click the Cytoscape app icon and select …/Cytoscape.app/Contents/vmoptions.txt file instead. Platform, the situation is slightly different – if you are launchingĬytoscape by clicking on the Cytoscape icon, you must edit the The fileĬontains one option per line, with each line terminated by a linefeed,Īnd an extra linefeed at the end of the file. Resides in the same directory as the Cytoscape executable. Maximum memory size by editing the Cytoscape.vmoptions file, which You can change Cytoscape’s initial and/or Variables – see your operating system documentation for furtherīy default, Cytoscape uses an estimate for initial and maximum memoryĪllocation based on your operating system, system architecture (32 or 64īit), and installed memory. Your operating system may have other mechanisms for setting environment Option to the Cytoscape.vmoptions file or the _JAVA_OPTIONSĮnvironment variable, substituting the desired path as appropriate: Installed on a workstation, but the home directory is stored on aĬentral file server. to the desired directory – this is useful when Cytoscape is To change the user homeĭirectory from the default, one can set the Java environment variable The u/ directory signifies the user’s home directory, which variesįrom user to user and from platform to platform. Various libraries needed to run the application. The core CytoscapeĪpplication assumes this directory structure when looking for the For Cytoscape to work properly, all files shouldīe left in the directory in which they were unpacked. The p/ directory signifies the program directory, which varies from U/CytoscapeConfiguration/cytoscape3.props Preset networks as described in the embedded README.txt fileĬytoscape properties and program cache files More automation flexibility is available using other settings and pre-programmed response files, as described in Appendix A of the Install4j manual ().Ĭytoscape installations (regardless of platform) contain theįollowing files and directories: Cytoscape files and directories Directory / FileĬytoscape program files, startup scripts, and default location for session files With a “-q” parameter, the installation package will automatically choose all default settings. For this to succeed, your execution environment must already have sufficient privileges to install software (e.g., for Windows: administrator priveleges). The installation process can be automated and made silent by executing the installation package with the “-q” command parameter (e.g., “Cytoscape_3_8_0-RC1_windows_64bit.exe -q”) from a command line or script. This will bring up a wizard that will lead you through the process, presenting choices for the installation directory, license agreement, file associations and privacy settings. The easiest and most common way to install Cytoscape is by executing an automatic installation package downloaded from the Cytoscape web site.
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